Structural Variation | Azwad Iqbal – Population Geneticist & Evolutionary Biologisthttps://azwadriqbal.github.io/tags/structural-variation/Structural VariationHugoBlox Kit (https://hugoblox.com)enFri, 07 Aug 2026 00:00:00 +0000https://azwadriqbal.github.io/media/logo.svgStructural Variationhttps://azwadriqbal.github.io/tags/structural-variation/BLink-seq delivers population-scale haplotypes without long reads: a scalable framework for non-model genomicshttps://azwadriqbal.github.io/publications/blinkseq/Fri, 07 Aug 2026 00:00:00 +0000https://azwadriqbal.github.io/publications/blinkseq/<p>BLink-seq is a linked-read sequencing library-preparation method that runs on standard short-read sequencers using low-cost reagents and scales to high-throughput sample processing. We tuned library-prep parameters against linked-read quality metrics, then tested phasing and structural-variant detection at two evolutionary extremes: an inbred <em>Drosophila melanogaster</em> cross carrying known inversions, and four wild-caught Atlantic silverside (<em>Menidia menidia</em>) parent-offspring trios. Applying the protocol across 376 silversides, we recovered chromosome-scale phased blocks, recovered the known inversions in both validation sets, and uncovered previously undescribed structural complexity inside an adaptive inversion on silverside chromosome 11.</p> <p>Documentation and a user guide are available at .</p>